Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Pdf isolation of phosphate solubilizing fungi from. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphate solubilizing bacteria, based upon visual observation. Isolation and characterization of a phosphatesolubilizing. Isolation of phosphate solubilizing fungi the isolation of phosphate solubilizing fungi from each type of oil palm empty fruit bunches was done by the pouring method on pikovskaya 1948 medium with tricalcium phosphate ca 3 po 3 2 as an insoluble organic. Now, it is important to identify betterfunctioning psolubilizing fungi. In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as talaromyces aurantiacus.
One hundred and forty five fungi isolates were incubated on. Inorganic phosphate solubilization is one of the major mechanisms of plant growth promotion by plant associated bacteria. Pigeon pea cajanus cajan is an important food crop, a principal source of protein in the indian diet, and a very popular food in developing tropical countries. Phosphatesolubilizing microorganisms have been considered as a novel alternative approach to provide phosphate fertilizers that promote plant growth. Islam et al isolation of phosphate solubilizing fungi from subtropical soils9145applied ecology and environmental research 174. Under the situation, many phosphate solubilizing microorganisms have been isolated from different environments and phosphorus availability to plants yu et al. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2 1department of plant science and technology, huazhong agricultural university, wuhan, hubei, p. Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. Isolation and screening of phosphate solubilizing bacteria wide number of bacterial isolates was found from the different agricultural lands of surat and bharuch district in gujarat, india. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Isolation and evaluation of rock phosphate solubilizing.
A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Those fungal colonies showing clear zone around the colonies were selected. Isolation and characterization of two phosphatesolubilizing fungi. Phosphate solubilization potential of rhizosphere fungi isolated. In this study, we first isolated two phosphate solubilizing fungi psf from moso bamboo rhizosphere soil in acid soil of jiangxi province, china. Phosphate solubilizing bacterial strain isolated from a jute mill effluent mixing area of ganga river water at bansberia 2258. Impact on growth and development of economically important plants. Isolation and charecterisation of seaweed endophytic fungi as. However, though results of this study make these fungi. Anantharaman cas in marine biology, faculty of marine sciences, annamalai university, parangipettai608502, tamil nadu, india. Exploration of phosphate solubilizing microorganisms has. Among them about 78 isolates was found from the different rhizosphere of sunflower plants.
Solubilization of inorganic phosphate by phosphate. Fungi isolation phosphate solubilizing fungi psf were isolated from 25 subsamples of the soils using selective media, pikovskaya medium pvk. One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium. New isolation method for microorganisms solulbilizing iron. This involves bacteria releasing organic acids into the soil which solubilize the phosphate complexes converting. Phosphatesolubilizing bacteria and fungi in various cultivated and virgin alberta soils. A total of twenty two rhizospheric fungi were isolated from different healthy crop plants of hassan, karnataka, india and tested for its efficacy to solubilize. The clear halo zone around the colonies was considered as positive result for psolubilization. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Isolation and characterization of phosphate solubilizing. Phosphate solubilizing bacteria are frequently used as plant growth promoters. Isolation and evaluation of rock phosphate solubilizing fungi as potential biofertilizer s. Research article phosphate solubilization potential of.
Mehta department of biotechnology, research and development centre, gsfc ltd vadodara, india abstract rock phosphate is the cheapest and abundant phosphatic fertilizer available but. The yield components of wheat and faba bean plants increased as a result of soil inoculation with the isolated fungi. In the present study, three phosphate solubilizing fungi c. Isolation of phosphate solubilizing fungi from rhizosphere soil and its. Interaction between phosphate solubilizing bacteria and. The analysis of phosphate solubilizing activity of the selected isolates were conducted qualitatively and quantitatively by plate screening method and broth culture method. Colony morphology of phosphorus solubilizing fungal. Phosphate solubilization potential of rhizosphere fungi. Phosphatesolubilizing bacteria are frequently used as plant growth promoters.
Now, it is important to identify betterfunctioning p solubilizing fungi. Isolation of phosphate solubilizing bacterial strain. Isolation of phosphate solubilizing microorganism psms from. Collected rhizosphere soil samples were used for the isolation of phosphate solubilizing fungi on pikovskayas pkv agar medium, containing the following gl. In the present study, three phosphatesolubilizing fungi c.
Impact of phosphatesolubilizing fungi on the yield and phosphorus. Phosphate solubilization all of the isolated bacterial isolates were checked for phosphate solubilization on pikoviskaya agar plates 1618. Isolation of phosphate solubilizing bacteria and fungi from rhizospheres soil from banana plants and its effect on the growth of amaranthus cruentus l. Plant growth promotion induced by phosphate solubilizing. Phosphatesolubilizing microorganisms associated with the. Isolation of psolubilizing fungi was carried out by serial dilution and pour plate technique. Isolation and characterization of two phosphatesolubilizing. Isolation of phosphate solubilizing fungi phosphate solubilizing fungi psf were isolated from each sample by serial dilution and spread plate method gaur, et al. Additionally sayer and gadd 1997, certain fungi are able to immobilize and release p by precipitating insoluble metal. Isolation and indentification of phosphate solubilizing fungi.
Therefore, this study aimed to isolate zincphosphatesolubilizing fungi zpsf from rhizospheric soil cultivated with economic crops. A study was conducted to isolate phosphatesolubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. Aug 25, 2018 phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Based on the result of in vitro studies, the inoculation effect of phosphate solubilizing microorganisms psms isolates on the nodulation, growth and phosphorus content of chickpea plants was studied under pot culture conditions using a efficient strain of pseudomonas sp. Mehta department of biotechnology, research and development centre, gsfc ltd vadodara, india abstract rock phosphate is the cheapest and abundant phosphatic fertilizer available but due to its sparse solubility. A total of 59 fungi were originally obtained and were screened at the. The collected soil samples were serially diluted using sterile water blanks and plated on rose bengal agar medium. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Solubilization of inorganic phosphate by rhizospheric fungi. A study was conducted to isolate phosphate solubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. Isolation of phosphate solubilizing bacteria and fungi from. Isolation and evaluation of rock phosphate solubilizing fungi as. Pdf isolation and characterization of two phosphate. Percentage of silicate solubilizing bacteria was checked for each soil sample and calculated as follows.
Isolation and characterization of phosphatesolubilizing. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement. This research is to isolate and select phosphate solubilizing soil fungi from kanchanaburi area. Pdf isolation of phosphate solubilizing fungi from rhizosphere soil. The two psf, talajx04 and aspnjx16, were identified as talaromyces aurantiacus and aspergillus neoniger respectively, based on morphologic and phylogenetic characteristics. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. In this study, three strains were isolated and identified as penicillium oxalicum fjg21, penicillium oxalicum fjq5, and bacillus subtilis bpm12, with a relatively high phosphatesolubilizing activity. Fungi and bacteria have the ability to solubilizing these compounds 4 isolation of microbial strains. The serially dilution from 10 to 105 was achieved by trans ferring 1ml of mushroom residue solution from each preceding attenuation stage to the next.
However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Isolation and identification of potential phosphate. Contrasting nutrient agar and calcium phosphate mediums represented the much higher mpn value on. Isolation and screening of silicate bacteria from various. Soil bacteria and fungi mediate soil processes such as decomposition, nutrient mobilization and mineralization. The screening of phosphate solubilizing fungi on pkv media was done after incubation, the observed colonies were morphologically distinct, both with and without halos. Optimization for rock phosphate solubilization by phosphate. Pdf identification of phosphatesolubilizing microorganisms. Isolation and indentification of phosphate solubilizing fungi from rhizosphere soil free download as pdf file. Because of the negative charge of phosphate ions, they are quickly absorbed after weathering of clays or detritus particles, forming insoluble forms of aluminum, calcium, or iron phosphates, all unavailable to mangroves. Lecturer5 department of microbiology malanakara catholic college mariagiri, kaliakkavilai 629053. Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. Chapter 3 isolation, screening, morphological and biochemical. E solubilization diameter growth diameter x 100 by 15.
Isolation of phosphate solubilizing fungi from rhizosphere soil and its effect on seed growth parameters of different crop plants article pdf available november 2016 with 583 reads how we. A number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Pdf phosphate solubilizing fungi isolated and characterized from. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement, especially in plimiting regions. The phosphate solubilizing efficiency of isolated psb strain kupsb12 in pikovskayas broth indicated that the strain efficiently solubilized inorganic phosphate in the medium containing 0. Phosphate solubilizing fungi isolated and characterized from teff. Isolation and charecterisation of seaweed endophytic fungi as an efficient phosphate solubiizers noorjahan a, b. Isolation screening and selection of phosphate solubilizing. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. The two psf, talajx04 and aspnjx16, were identified as talaromyces aurantiacus and aspergillus neoniger respectively, based on. In this study, we first isolated two phosphatesolubilizing fungi psf from moso bamboo rhizosphere soil in acid soil of jiangxi province, china.
Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphatesolubilizing bacteria, based upon visual observation. Among the whole microbial population in soil, psb constitute 1 to 50 %, while phosphorus solubilizing fungi psf are only 0. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. The use of plant growth promoting bacterial inoculants as live microbial biofertilizers provides a promising alternative to chemical fertilizers and pesticides. After screening, 14 fungal isolates were obtained, which were further screened based. Isolation and characterization of two phosphatesolubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and ph environments. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. Number of psb among total psm in north iranian soil was around 88 % fallah, 2006. Under the situation, many phosphatesolubilizing microorganisms have been isolated from different environments and phosphorus availability to plants yu et al. Isolation, screening and characterization of phosphate. Arbuscular mycorrhizal fungi amf and phosphate solubilizing bacteria psb could interact synergistically because psb solubilize sparingly available phosphorous compounds into orthophosphate. Request pdf isolation and evaluation of rock phosphate solubilizing fungi as potential biofertilizer rock phosphate is the cheapest and abundant phosphatic fertilizer available but due to its. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l.
Isolation of phosphate solubilizing bacteria and fungi. Impact of heavy metals and initial ph variations on the. After confirming the phosphate solubilizing ability on solid medium, the phosphate solubilization were. Many bacteria and fungi are able to improve plant growth by solubilizing inorganic and organic phosphates in the soil. Isolation and characterization of bacteria and fungi from. The phosphate solubilization ability of the fungi was analyzed by determining the phosphate solubilization efficiency e of each isolate. Phosphate solubilizing fungi isolated and characterized from teff rhizosphere soil collected from north showa zone, ethiopia. Isolation and identification of phosphate solubilizing.
Isolation of phosphate solubilizing microorganism psms. Pikovskayas agar amended with tricalcium phosphate was used to isolate the psolubilizing fungi. Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. Isolation of phosphorussolubilizing fungus from soil to. Phosphorus p is one of the major bioelements limiting agricultural production. Therefore, this study aimed to isolate zinc phosphate solubilizing fungi zpsf from rhizospheric soil cultivated with economic crops. Screening, isolation, biochemical and plant growth promoting.
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